@article{186431,
  keywords = {Animals, Protein Binding, Amino Acid Sequence, Cell Communication, Female, Male, Guanosine Triphosphate, Actins, Drosophila melanogaster, Endocytosis, Sequence Homology, Actin Cytoskeleton, Dynamins, Actin-Related Protein 2-3 Complex, Myoblasts},
  author = {Ruihui* Zhang and Donghoon Lee and John Jimah and Nathalie Gerassimov and Changsong Yang and Sangjoon Kim and Delgermaa Luvsanjav and Jonathan Winkelman and Marcel Mettlen and Michael Abrams and Raghav Kalia and Peter Keene and Pratima Pandey and Benjamin Ravaux and Ji Hoon Kim and Jonathon Ditlev and Guofeng Zhang and Michael Rosen and Adam Frost and Neal Alto and Margaret Gardel and Sandra Schmid and Tatyana Svitkina and Jenny Hinshaw and Elizabeth Chen},
  title = {Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein},
  abstract = {<p>The dynamin GTPase is known to bundle actin filaments, but the underlying molecular mechanism and physiological relevance remain unclear. Our genetic analyses revealed a function of dynamin in propelling invasive membrane protrusions during myoblast fusion in vivo. Using biochemistry, total internal reflection fluorescence microscopy, electron microscopy and cryo-electron tomography, we show that dynamin bundles actin while forming a helical structure. At its full capacity, each dynamin helix captures 12-16 actin filaments on the outer rim of the helix. GTP hydrolysis by dynamin triggers disassembly of fully assembled dynamin helices, releasing free dynamin dimers/tetramers and facilitating Arp2/3-mediated branched actin polymerization. The assembly/disassembly cycles of dynamin promote continuous actin bundling to generate mechanically stiff actin super-bundles. Super-resolution and immunogold platinum replica electron microscopy revealed dynamin along actin bundles at the fusogenic synapse. These findings implicate dynamin as a unique multifilament actin-bundling protein that regulates the dynamics and mechanical strength of the actin cytoskeletal network.</p>
},
  year = {2020},
  journal = {Nat Cell Biol},
  volume = {22},
  pages = {674-688 (* co-first authors)},
  month = {06/2020},
  issn = {1476-4679},
  doi = {10.1038/s41556-020-0519-7},
  language = {eng},
}